 MARCATORI GENETICI INVESTIGATIVI
Per la determinazione dei profili genetici viene utilizzato il kit AmpFlSTR®
Identifiler™ prodotto dalla Applied Biosystems, mediante il quale vengono amplificate
e genotipizzate le seguenti regioni STR:
HumTH01 (HOLGERSSON et al., Electrophoresis
1994, 15, 890-895);
D19S433 (Cooperative Human Linkage Center,
accession nr. 135, GeneBank accession nr. G08036);
HumvWA (Kimpton C.P. et al., 1992, Hum.
Mol. Genet. Vol. 1 pag. 287);
D21S11 (SHARMA & LITT, 1992 Hum Mol
Genet 1:67, GILL et al. 1997; For Sci Int 86:25).
HumFGA (MILLS, K.A. et al. 1992, Human
Molecular Genetics 1:779);
D18S51 (URQUHART et al. BioTechniques Vol.
18, n. 1 1995);
D16S539 (Cooperative Human Linkage Center,
accession nr. 715, GeneBank accession nr. G07925);
D8S1179 (OLROYD te al. 1995;
Electrophoresis 16:349);
D5S818;
D7S820;
D13S317
D3S1358
CSF1PO (HAMMOND et al. 1994; Am J Hum Genet
55: 175);
TPOX (ANKER et al. 1992; Am J Hum Genet 55:
175);
D2S1338 (Cooperative Human Linkage Center,
accession nr. 41445, GeneBank accession nr. G08202).
Amelogenina ()
Locus |
Localizzazione cromosomica |
Alleli |
(PE) |
| D8S1179
|
8 |
8, 9
10, 11, 12, 13,14, 15, 16, 17, 18, 19 |
0.680 |
| D21S11
|
21q11.2-q21 |
24,
24.2, 25, 26, 27,28, 28.2, 29, 29.2,30, 30.2, 31, 31.2,32, 32.2, 33, 33.2,34, 34.2, 35,
35.2,36, 37, 38 |
0.708 |
| D7S820
|
7q11.21-22 |
6, 7,
8, 9, 10, 11, 12,13, 14, 15 |
0.582 |
| CSF1PO |
5q33.3-34 |
6, 7,
8, 9, 10, 11, 12,13, 14, 15 |
0.496 |
| D3S1358
|
3p |
12, 13,
14, 15, 16,17, 18, 19 |
0.630 |
| TH01
|
11p15.5 |
4, 5,
6, 7, 8, 9, 9.3,10, 11, 13.3 |
0.566 |
| D13S317
|
13q22-31 |
8, 9,
10, 11, 12, 13,14, 15 |
0.487 |
| D16S539
|
16q24-qter |
5, 8,
9, 10, 11, 12,13,14, 15 |
0.566 |
| D2S1338
|
2q35-37.1 |
15, 16,
17, 18, 19,20, 21, 22, 23, 24,25, 26, 27, 28 |
0.725 |
| D19S433
|
19q12-13.1 |
9, 10,
11, 12, 12.2,13, 13.2, 14, 14.2,15, 15.2, 16, 16.2,17, 17.2 |
0.531 |
| vWA |
12p12-pter |
11,12,
13, 14, 15, 16,17, 18, 19, 20, 21,22, 23, 24 |
0.625 |
| TPOX
|
2p23-2per |
6, 7,
8, 9, 10, 11, 12,13 |
0.329 |
| D18S51
|
18q21.3 |
7, 9,
10, 10.2, 11, 12,13, 13.2, 14, 14.2,15, 16, 17, 18, 19,20, 21, 22, 23, 24,25, 26, 27 |
0.731 |
| D5S818
|
5q21-31 |
7, 8,
9, 10, 11, 12,13, 14, 15, 16 |
0.440 |
| FGA |
4q28 |
17, 18,
19, 20, 21,22, 23, 24, 25, 26,26.2, 27, 28, 29, 30,30.2, 31.2, 32.2,33.2, 42.2, 43.2,44.2,
45.2, 46.2,47.2, 48.2, 50.2,51.2 |
0.766 |
| Amelogenin
X/Y |
Xp22.1-22.3/ Yp11.2 |
X, Y |
|
| |
|
Combined (PE) |
0.9999992 |
(PE) = Probabilità di esclusione.
|
